Identification of a unique isoform of 1-aminocyclopropane-1-carboxylic acid synthase by monoclonal antibody
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence of a cDNA clone encoding 1-aminocyclopropane-1-carboxylic acid synthase from broccoli.
Ethylene regulates many processes in plant growth, development, and senescence. Two enzymes, ACC synthase and ACC oxidase, are responsible for ethylene biosynthesis from its precursor S-adenosyl Met (Yang and Hoffman, 1984), and blocking the synthesis of these enzymes in antisense RNA experiments results in reduced ethylene synthesis (Hamilton et al., 1990; Oeller et al., 1991). Functional iden...
متن کاملSynthesis of Deuterium - labeled 1 - Aminocyclopropane - 1 - carboxylic Acid
[ ZH2 IACC) ; 1-amino [ 2R, 3E-2H2] cyc lopropaneIcar boxy 1 ic acid (trans-[zH2]ACC) : and l-amino[2,2.3.3-~H~]cyclopropane1-carboxylic acid are described. The [2,2-2H2]and [2,2,3.3-2H4]ACC compounds were prepared from the appropriately deuterated 2-bromoethanol 4-methylbenzenesulfonates by reaction with ethyl isocyanoacetate and two moles oE sodium hydride. The transr2H2]ACC and &[2H2]ACC wer...
متن کاملIsolation and Molecular Characterization of 1-Aminocyclopropane-1-carboxylic Acid Synthase Genes in Hevea brasiliensis
Ethylene is an important factor that stimulates Hevea brasiliensis to produce natural rubber. 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is a rate-limiting enzyme in ethylene biosynthesis. However, knowledge of the ACS gene family of H. brasiliensis is limited. In this study, nine ACS-like genes were identified in H. brasiliensis. Sequence and phylogenetic analysis results confirmed t...
متن کاملThe involvement of 1-aminocyclopropane-1-carboxylic acid synthase isogene, Pp-ACS1, in peach fruit softening.
Ethylene promotes fruit ripening, including softening. The fruit of melting-flesh peach (Prunus persica (L). Batsch) cultivar 'Akatsuki' produces increasing levels of ethylene, and the flesh firmness softens rapidly during the ripening stage. On the other hand, the fruit of stony hard peach cultivars 'Yumyeong', 'Odoroki', and 'Manami' does not soften and produces little ethylene during fruit r...
متن کاملEthylene-Enhanced 1-Aminocyclopropane-1-carboxylic Acid Synthase Activity in Ripening Apples.
Apples (Malus sylvestris Mill, cv Golden Delicious) were treated before harvest with aminoethoxyvinylglycine (AVG). AVG is presumed to reversibly inhibit 1-aminocyclopropane-1-carboxylic acid (ACC) activity, but not the formation of ACC synthase. AVG treatment effectively blocked initiation of autocatalytic ethylene production and ripening of harvested apples. Exogenous ethylene induced extract...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1988
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.85.23.8810